ABSTRACT
The quantification of the antibody response to SARS-CoV-2 vaccination is considered relevant for identifying possible vaccine failure and estimating protection time. Various types of vaccines have been produced to suppress the development of SARS-Cov2 from numerous approaches, starting from the use of inactivated whole viruses, genetic material (mRNA), and transmembrane proteins (spike protein-RDB). The strategy to increase herd immunity to SARS-cov2 infection through the vaccination program is estimated to impact health significantly. The presence of post-vaccination antibodies (IgG) needs to be monitored to determine the effectiveness of vaccines that have been immunized on a person, how long the protective power is produced from vaccinations that a person receives so that other vaccination programs can be confirmedly determined to obtain herd immunity and eliminate transmission of COVID-19 nationally. This study aimed to study the polyclonal character of the antibody induced by the specific protein from post-vaccinated patients. In this study, we also determined the highest antibody titer among groups using the ELISA method. According to SDS-PAGE visualization, we found a specific band, a 158 kDa, found explicitly in post-vaccine groups only. This protein will be selected as a protein antigen to produce rabbit polyclonal antibodies for further study. Based on the results of the polyclonal antibody production, it can be concluded that a 158 kDa protein isolate can stimulate a humoral immune response by a polyclonal antibody with the highest antibody titer detected at fifth-week blood collection. The selected polyclonal antibody specificity test was able to identify protein isolates from post-vaccinated patient's serum with a molecular weight of 158 kDa. © 2023 American Institute of Physics Inc.. All rights reserved.
ABSTRACT
OBJECTIVES To assess the knowledge and personal hygiene behavior among students in Islamic boarding schools regarding the prevention and transmission of COVID-19, and correlate with the findings of the COVID-19 screening program. METHOD A cross-sectional study was carried out during January to March 2021 using a validated questionnaire. The knowledge and personal hygiene behavior were interpreted as good, moderate, and poor. The screening of COVID-19 was performed using the antibody rapid test. The association between the screening of COVID-19 and the degree of knowledge and personal hygiene behavior was assessed using multiple logistic regression. RESULTS Of a total of 294 participants in the study, ranging in age between 12 and 17 years, 202 (68.7%) were males. Of these, 18 tested positive for COVID-19 by rapid test. Overall, 61.2% participants had moderate knowledge regarding COVID-19 prevention, but we failed to show correlation between knowledge and the transmission of COVID-19 among the pupils. Good personal hygiene behavior was recorded in 41.8% participants, but its correlation with the transmission of COVID-19 was not clarified. CONCLUSIONS The majority of our boarding school student sample had good-moderate knowledge of COVID-19 prevention and adequate personal hygiene behavior. The transmission of COVID-19, however, in our participants was not correlated with their knowledge and personal hygiene behavior. Copyright © Athens Medical Society.
ABSTRACT
The COVID-19 pandemic has changed the learning environment at all levels of education. Online learning has been running for more than a year. This makes all students must be able to adapt to the learning method as much as possible. Especially for Electrical Engineering students who should have practicum activities in learning Analog Electronics courses. Due to limited access to the lab, the practicum activities for this course are simulated on a computer. In the remote practicum process, students need to install the application and its environment. This learning becomes an obstacle when students cannot do the installation properly, especially for students who do not have adequate personal computer specifications. The use of Virtual Machine (VM) Collaboration as an alternative step to overcome these problems by giving computer access rights to students. Computers that have installed applications that are used for Analog Electronics practicum and assignments. Thus accelerating the initial setup and learning activities can run more focused on increasing student competence. The design of VM media has four steps as follows: 1) identifying laboratory activities, 2) exploring platforms and tools, 3) creating classwork/assignments, and 4) evaluating the media learning. The results of this research are in the form of a practicum job sheet and a web server as an information center and coordinating the use of VM Collaboration. In further research, to increase the use of VM Collaboration, it can be integrated with student access such as SIAKAD. This makes it easier for students to access computers virtually and is more flexible. © 2021 IEEE.
ABSTRACT
This study discusses the design and development of learning resources for online analog electronics courses during Covid-19 at D3 Electronic Engineering, Univeristas Negeri Malang. The online course becomes an obstacle when students cannot install and operate the software properly due to a lack of knowledge and limited infrastructure. It takes learning media that can be accessed and operated easily. Job sheet ThinkerCad development is one solution to create structured practicum activities. Tinkercad is a website-based virtual laboratory. Tinkercad provides classes where teachers can monitor and check student simulations so that it is like doing a practicum in a laboratory. The proposed approach has four steps: 1) identifying laboratory activities, 2) exploring platforms and tools, 3) creating classwork/assignments, and 4) evaluating media. The Job sheets consist of two types, namely for students and teachers. It also develops a manual book for the beginner. There is a discussion of the worksheet on the teacher's job sheet. The job sheet consists of the clipper, clamper, rectifier, voltage multiplier, transistor as switch and amplifier, class B and AB amplifier, and comparator. Based on the test results of the job sheet, there is a difference of about 10% between the theoretical calculation results with the simulation results. Future research will focus on the effectiveness of using Tinkercad in the Analog Electronics course. © 2021 IEEE.
ABSTRACT
This study was to see how to optimize the application and laboratory software on Oscillation and Wave material. This study was conducted using computers and android equipped with Internet access in SAMR framework. SAMR is a framework that requires the use of technology in learning The design of this research is content analysis. The results show that the Olabs application is complete and the most capable of explaining the formula if it is reviewed mathematically and physis meaning. This application is also able to explain the oscillator and wave phenomena well. Based on this research, during the Covid-19 pandemic, students can do STEM (science, technology, engineering and mathematics) from home, one of which is with the help of the Olabs and PhET application.